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KMID : 0613820080180070992
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Journal of Life Science
2008 Volume.18 No. 7 p.992 ~ p.998
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Establishment of the Extraction Process by Evaluation of Proanthocyanidin Contents and Antioxidative Activities of Pine (Pinus densiflora) Needle Extracts
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Lee Ok-Hee
Kim Ki-Young
Jang Min-Kyung
Yoo Ki-Hwan
Yoo Byung-Hong
Kim Sung-Gu
Kim Mi-Hyang
Lee Sang-Hyun
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Abstract
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We evaluated proanthocyanidin contents out of total polyphenolic compounds in two pine needle extracts prepared by combined extraction methods and compared their antioxidative activities. Amounts of total polyphenolic compounds of sub-supercritical hotwater precipitate (SSHP) and sub-supercritical hotwater supermatant (SSHS) were 225.4 and 116.6 ㎎/g, respectively. Amounts of proanthocyanidin of SSHP and SSHS were 9.9 and 3.6 ㎎/g, respectively. Thus, the ratio of proanthocyanidin contents over total polyphenolic compounds of SSHP and SSHS were 4.3 and 3.9%, respectively. Antioxidative activities (IC50) of SSHP and SSHS measured by DPPH radical scavenging were 41.5 and 50.7 ㎍/ml, respectively. These results indicates that two combined extracts, SSHP and SSHS showed higher antioxidative activities than extract prepared by hot water or sub-supercritical. In conclusion, extraction procedures for SSHP and SSHS could be useful methods for preparing the effective components of functional food products.
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KEYWORD
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Antioxidativeactivity, pineneedle, proanthocyanidin, totalphenoliccompound
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